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. 2022 Jun 14;12:9897. doi: 10.1038/s41598-022-13812-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of cryopreservation on cells dissociated using the one-step collagenase protocol in FACS and scRNA-seq. (A) Boxplots showing FACS cell viability (%) in dissociated cells from fresh versus cryopreserved samples for whole biopsy (one-step collagenase protocol) and fraction 2 (three-step protease protocol) (p-value > 0.05; non-paired t-test). Sample size is depicted in the lower part of the plot. UMAPs of scRNA-seq data obtained from two-paired fresh and cryopreserved samples dissociated with one-step collagenase colored by (B) cell types and (C) storage condition. (D) Bar plot of relative cell type frequencies recovered from the scRNA-seq data colored by cell-type.