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. 2022 Jun 14;12:9897. doi: 10.1038/s41598-022-13812-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Decision tree for single-cell dissociation of gut tissue for scRNA-seq. (A) Flow-chart designed to assist researchers in choosing a dissociation protocol for gut tissue based on their specific needs. (B) Overview of the main differences between the protocols. IEL, intraepithelial cell; EC, epithelial cell; EL, epithelial layer; LP, lamina propria; F1, fraction 1; F2, fraction 2.