| Antibodies |
| Chicken Anti-GFP |
Abcam |
Cat# ab13970 |
| BV421 Rat Anti-CD4 (GK1.5) |
BD Biosciences |
Cat# 562891 |
| BV786 Rat Anti-CD8 (53-6.7) |
BD Biosciences |
Cat# 563332 |
| BV786 Rat Anti-CD25 (PC61) |
BD Biosciences |
Cat# 564023 |
| BV711 Hamster Anti-CD69 (H1.2F3) |
BD Biosciences |
Cat# 740664 |
| Rat Anti-Mouse CD16/CD32 Fc Block (2.4G2) |
BD Biosciences |
Cat# 553141 |
| Alexa Fluor 647 Rat anti-FoxP3 (MF23) |
BD Biosciences |
Cat# 560401 |
| Mouse Anti-Smooth Muscle Actin (1A4) |
Biocare Medical |
Cat# 001 |
| Rat IgG2a Isotype Control (2A3) |
BioXCell |
Cat# BE0089 |
| PE Anti-Ly6C (HK1.4) |
Biolegend |
Cat# 128008 |
| Rat Anti-CD74 (CLIP) |
Biolegend |
Cat# 151002 |
| Brilliant Violet 421 Rat Anti-I-A/I-E (M5/114.15.2) |
Biolegend |
Cat# 107632 |
| PE Anti-IL-6 (MP5-20F3) |
Biolegend |
Cat# 504504 |
| PE Anti-Ki-67 (16A8) |
Biolegend |
Cat# 652404 |
| APC Anti-Podoplanin (8.1.1) |
Biolegend |
Cat# 127410 |
| Rabbit Phospho-NF-κB p65 (Ser536) (93H1) |
Cell Signaling |
Cat# 3033S |
| Rabbit Anti-CD74 (D5N3I) |
Cell Signaling |
Cat# 77274S |
| Rabbit Anti-CD8 (D4W2Z) |
Cell Signaling |
Cat# 98941S |
| Rabbit Anti-PDGFRα (D13C6) |
Cell Signaling |
Cat# 5241S |
| Mouse Anti-Smad2 (L16D3) |
Cell Signaling |
Cat# 3103S |
| Rabbit Anti-Phospho-Smad2 (Ser465/467) (138D4) |
Cell Signaling |
Cat# 3108S |
| Mouse Anti-CD3 |
Invitrogen |
Cat# PA1-29547 |
| Peroxidase AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) |
Jackson ImmunoResearch |
Cat# 703-035-155 |
| Peroxidase AffiniPure Donkey Anti-Mouse IgG (H+L) |
Jackson ImmunoResearch |
Cat# 715-035-150 |
| Peroxidase AffiniPure Donkey Anti-Rabbit IgG (H+L) |
Jackson ImmunoResearch |
Cat# 711-035-152 |
| Peroxidase AffiniPure Goat Anti-Syrian Hamster IgG (H+L) |
Jackson ImmunoResearch |
Cat# 107-035-142 |
| Rabbit Anti-Cadherin-11 |
LSBio |
Cat# LS-B2308 |
| Rabbit Anti-IL-6 |
LSBio |
Cat# LS-C809503 |
| Rabbit Anti-Mesothelin |
LSBio |
Cat# LS-C407883 |
| Rat Anti-Mesothelin (B35) |
MBL Bio |
Cat# D233-3 |
| Rabbit Anti-Actin |
MilliporeSigma |
Cat# A2066 |
| Syrian Hamster Anti-Podoplanin (8.1.1) |
MilliporeSigma |
Cat# MABT1512 |
| Rabbit Anti-Sox9 |
MilliporeSigma |
Cat# AB5535 |
| Goat Anti-tdTomato |
MyBioSource |
Cat# MBS448092 |
| Rabbit Anti-FoxP3 |
R&D Systems |
Cat# MAB8214 |
| Rabbit Anti-NFκB p65 |
Santa Cruz |
Cat# sc-109 |
| Alexa 488 Anti-Smooth Muscle Actin (1A4) |
Thermo Fisher Scientific |
Cat# 53-9760-82 |
| Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 |
Thermo Fisher Scientific |
Cat# A-11030 |
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 |
Thermo Fisher Scientific |
Cat# A-11035 |
| Bacterial and Virus Strains |
| pLV-eGFP |
Addgene |
Cat# 36083 |
| Chemicals, Peptides, and Recombinant Proteins |
| Recombinant Mouse EGF |
Biolegend |
Cat# 585608 |
| OVA Peptide (323-339) |
GenScript |
Cat# RP10610 |
| Collagenase XI |
MilliporeSigma |
Cat# C7657 |
| DNase I |
MilliporeSigma |
Cat# D4527 |
| Hyaluronidase |
MilliporeSigma |
Cat# H3506 |
| Hydrocortisone |
MilliporeSigma |
Cat# H0888 |
| Tamoxifen |
MilliporeSigma |
Cat# T5648 |
| Recombinant Human TGFβ1 (CHO derived) |
PeproTech |
Cat# 100-21C |
| Recombinant Mouse IL-1 alpha/IL-1F1 Protein |
R&D Systems |
Cat# 400-ML-005/CF |
| Gibco Dispase |
Thermo Fisher Scientific |
Cat# 17-105-041 |
| Collagenase IV |
Thermo Fisher Scientific |
Cat# 17104019 |
| Collagenase I |
Worthington Biochemical |
Cat# LS004196
|
| Collagenase II |
Worthington Biochemical |
Cat# LS004176
|
| Collagenase III |
Worthington Biochemical |
Cat# LS004182
|
| Elastase |
Worthington Biochemical |
Cat# LS002279
|
| Critical Commercial Assays |
| Fixable Viability Stain |
BD Biosciences |
Cat# 564406 |
| Mouse FoxP3 Buffer Set |
BD Biosciences |
Cat# 560409 |
| MojoSort mouse CD4 T cell isolation kit |
Biolegend |
Cat# 480033 |
| MojoSort mouse CD8 T cell isolation kit |
Biolegend |
Cat# 480035 |
| iScript cDNA Synthesis Kit |
Bio-Rad |
Cat# 1708891 |
| iTaq Universal SYBR® Green Supermix |
Bio-Rad |
Cat# 1725121 |
| ProLong Gold Antifade Reagent with DAPI |
Cell Signaling |
Cat# 8961S |
| Senescence β-Galactosidase Staining Kit |
Cell Signaling |
Cat# 9860S |
| Opal 520 Reagent Pack |
PerkinElmer |
Cat# FP1487001KT |
| Opal 570 Reagent Pack |
PerkinElmer |
Cat# FP1488001KT |
| Opal 620 Reagent Pack |
PerkinElmer |
Cat# FP1495001 KT |
| Opal 690 Reagent Pack |
PerkinElmer |
Cat# FP1497001 KT |
| RNeasy Mini Kit |
Qiagen |
Cat# 74106 |
| CellTrace CFSE Cell Proliferation Kit |
Thermo Fisher Scientific |
Cat# C34554
|
| BCA Protein Assay Kit |
Thermo Fisher Scientific |
Cat# 23225 |
| SuperSignal West Pico PLUS Chemiluminescent Substrate |
Thermo Fisher Scientific |
Cat# 34577 |
| ImmPRESS AP Horse Anti-Rabbit IgG Polymer Detection Kit |
Vector Laboratories |
Cat# MP-5401 |
| ImmPRESS AP Horse Anti-Mouse IgG Polymer Detection Kit |
Vector Laboratories |
Cat# MP-5402 |
| ImmPRESS HRP Horse Anti-Goat IgG Polymer Detection Kit |
Vector Laboratories |
Cat# MP-7405 |
| ImmPRESS HRP Goat Anti-Mouse IgG Polymer Detection Kit |
Vector Laboratories |
Cat# MP-7452 |
| ImmPRESS HRP Goat Anti-Rabbit IgG Polymer Detection Kit |
Vector Laboratories |
Cat# MP-7451 |
| Biological Samples |
| Unstained paraffin-embedded tissue sections of PDA |
UTSW Tissue Management Shared Resource |
https://www.utsouthwestern.edu/departments/simmons/research/shared-resources/tissue-management.html
|
| Unstained paraffin-embedded tissue sections of PDA |
MD Anderson Institutional Tissue Bank |
https://www.mdanderson.org/research/research-resources/core-facilities/institutional-tissue-bank.html
|
| Experimental Models: Cell Lines |
| Mouse Cell Line: BMFA3 |
Huang et al., 2019
|
N/A |
| Mouse Cell Line: CT1BA5 |
Huang et al., 2019
|
N/A |
| Mouse Cell Line: PanMeso |
This Paper |
N/A |
| Mouse Cell Line: 6419c5 |
Li et al., 2018
|
N/A |
| Mouse Cell Line: 6694c2 |
Li et al., 2018
|
N/A |
| Mouse Cell Line: 6499c4 |
Li et al., 2018
|
N/A |
| Mouse Cell Line: 2838c3 |
Li et al., 2018
|
N/A |
| Mouse Cell Line: 6620c1 |
Li et al., 2018
|
N/A |
| Experimental Models: Organisms/Strains |
| Mouse: KIC (KrasLSL-G12D/+;Ink4afl/fl;Ptf1aCre/+) |
Hingorani et al., 2005
|
N/A |
| Mouse: KPfC (KrasLSL-G12D/+;Trp53fl/fl;Pdx1Cre/+) |
Hingorani et al., 2003
|
N/A |
| Mouse: C57BL/6J |
The Jackson Laboratory |
Cat# 000664 |
| Mouse: Immortomouse (Tg(H2-K1-tsA58)6Kio/LicrmJ |
The Jackson Laboratory |
Cat# 032619 |
| Mouse: OT II (B6.Cg-Tg(TcraTcrb)425Cbn/J) |
The Jackson Laboratory |
Cat# 004194 |
| Mouse: Wt1tm2(cre/ERT2)Wtp/J |
The Jackson Laboratory |
Cat# 010912 |
| Mouse: B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J |
The Jackson Laboratory |
Cat# 007914 |
| Deposited Data |
| Single-cell RNA sequencing |
Hosein et al., 2019
|
GEO: GSE125588
|
| Single-cell RNA sequencing |
Dominguez et al., 2020
|
ArrayExpress: E-MTAB-8483 |
| Single-cell RNA sequencing |
Elyada et al., 2019
|
GEO: GSE129455
|
| Single-cell RNA sequencing |
Lee et al., 2021
|
GEO: GSE156405
|
| Single-cell RNA sequencing |
Hornburg et al., 2021
|
EGA European Genome-Phenome Archive: EGAS00001004935 |
| Bulk RNA sequencing |
This Paper |
GEO: GSE196740
|
| Software and Algorithms |
| R package Seurat (v3.2.2) |
Butler et al., 2018
Stuart et al., 2019
|
N/A |
| R package Seurat (v4.0.4) |
Hao et al., 2021 |
N/A |
| R package Monocle2 (v2.16.0) |
Qiu et al., 2017
|
N/A |
| R language |
R-project |
https://www.R-project.org/
|
| FlowJo software (v10.7) |
BD Biosciences |
https://www.flowjo.com/
|
| Graphpad Prism 8.0 software |
GraphPad Software, Inc. |
http://www.graphpad.com/scientific-software/prism/
|
| ZEN Imaging Software |
Zeiss |
https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html
|
| Vectra Polaris Slide Scanner |
Akoya Biosciences |
https://resources.perkinelmer.com/lab-solutions/resources/docs/APP_Vectra-Polaris_013270_01.pdf
|
| Phenochart |
Akoya Biosciences |
https://www.akoyabio.com/support/software/phenochart-whole-slide-viewer/
|
| inForm |
Akoya Biosciences |
https://www.akoyabio.com/phenoimager/software/inform-tissue-finder/
|
| HALO image analysis software |
Indica Labs |
https://indicalab.com/news/indica-labs-release-halo-the-next-generation-image-analysis-platform/
|
| NDP.view2 software |
Hamamatsu Photonics |
https://www.hamamatsu.com/us/en/product/type/U12388-01/index.html
|
| Metascape |
Zhou et al., 2019
|
https://metascape.org/
|
