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. 2022 Jun 15;19:149. doi: 10.1186/s12974-022-02514-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Infection efficiency of SARS-CoV-2 analysis by RT-qPCR and immunofluorescence. A Infection efficiency of SARS-CoV-2 was detected using SARS-COV-2 Spike rabbit antibody, by immunofluorescence. Nuclei were stained in blue with DAPI, while SARS-CoV-2 was stained in red. Scale bar, 20 μm. B RT-qPCR for the RNA-dependent RNA polymerase (RdRP)-coding gene of SARS-CoV-2. Data are expressed as mean ± SD from three independent experiments. C Brain samples of both mice infected with SARS-CoV-2 for 5 d and those without were analyzed to determine the SARS-CoV-2 location by immunofluorescence. Nuclei were stained in blue with DAPI, while SARS-CoV-2 was stained in red. CD31 was specifically applied for labeling the micro-vessels in green. Scale bar indicates 50 μm