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Generation of a HEK293FT transgenic clone stably expressing pTK-CRISPRa-GFP
(A) Targeted integration of a donor pTK-CRISPRa-GFP into the genomic AAVS1 locus via CRISPR-Cas9-mediated homologous recombination. (B) Confirming site-specific integration of the full-length pTK-CRISPRa-GFP sequences at the genomic AAVS1 locus by PCR genotyping. AAVS1, adeno-associated virus integration site 1; GFP, green fluorescent protein; pTK; thymidine kinase promoter.
