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. 2022 May 29;26:26–37. doi: 10.1016/j.omtm.2022.05.005

Figure 5.

Figure 5

Activation of endogenous genes in HEK293FT cells by evolved CRISPRa

Targeted activation of endogenous human (A) KLOTHO, (B) SIRT6, and (C) NFE2L2 genes. Red arrows indicate the sense or antisense orientation of sgRNAs designed to recognize target DNA sequences on the promoter. Bottom panel shows the relative expression level of endogenous genes in HEK293FT cells. The statistical significance levels from VP64-WT are indicated as ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. All data are presented as mean ± SD (n = 3).