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. 2000 Apr;66(4):1298–1304. doi: 10.1128/aem.66.4.1298-1304.2000

FIG. 5.

FIG. 5

Plasmid profiles of E. faecalis BFE 1071 before and after plasmid curing. (a) Agarose gel electrophoresis of plasmids of E. coli V517 (lane 1), E. faecalis BFE 1071 (lane 2), mutant 1071/78 (lane 3), and mutant 1071/79 (lane 4). The numbers on the left indicate the sizes of the plasmids isolated from E. coli V517. (b) The same gel after Southern blotting onto a MagnaGraph nylon transfer membrane (MSI, Westboro, Mass.) and hybridized with a digoxigenin-labeled cloned EcoRV fragment of plasmid pEF1071 containing the genes encoding enterocins 1071A and 1071B.