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. 2022 Jun 1;10:875132. doi: 10.3389/fcell.2022.875132

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Copyright © 2022 Shah, McGuigan, Cheng, Vanpouille-Box, Demaria, Weiss and Lammerding.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of ATM but not ATR or DNA-PK reduces lamin A levels. (A) Representative immunoblot for lamin A and lamin C of MDA-MB-231 and HT1080 cells treated with 10 µM of ATM inhibitor KU-55933 or 0.1% DMSO for 48 h. Histone H3 was used as loading control. (B) Quantification of lamin A and C protein levels from immunoblots of MDA-MB-231 cells, based on three independent experiments. Lamin levels were normalized to DMSO treated cells. *, p < 0.05 based on Two-way ANOVA with Tukey’s multiple comparison test. N = 3 (C) Quantification of lamin A and C protein levels from immunoblots of HT1080 cells, based on three independent experiments. Lamin levels were normalized to DMSO treated cells. *, p < 0.05 based on Two-way ANOVA with Tukey’s multiple comparison test. N = 3 (D) Representative immunoblot for lamin A and lamin C of MDA-MB-231 and HT1080 cells treated with 10 µM of ATR inhibitor VE-821 or 0.1% DMSO for 48 h. Histone H3 was used as loading control. (E) Quantification of lamin A and C protein levels from immunoblots for MDA-MB-231 cells, based on three independent experiments. Lamin levels were normalized to DMSO treated cells. Differences were not statistically significant (n.s.) based on two-way ANOVA. N = 3 (F) Quantification of lamin A and C protein levels from immunoblots for HT1080 cells, based on three independent experiments. Lamin levels were normalized to DMSO treated cells. Differences were not statistically significant (n.s.) based on two-way ANOVA. N = 3 (G) Representative immunoblot for lamin A and lamin C of MDA-MB-231 and HT1080 cells treated with 5 µM of DNA-PK inhibitor NU7441 or 0.1% DMSO for 48 h. Histone H3 was used as loading control. (H) Quantification of lamin A and C protein levels from immunoblots for MDA-MB-231 cells, based on three independent experiments. Lamin levels were normalized to DMSO treated cells. Differences were not statistically significant (n.s.) based on two-way ANOVA. N = 3. (I) Quantification of lamin A and C protein levels from immunoblots for HT1080 cells, based on three independent experiments. Lamin levels were normalized to DMSO treated cells. Differences were not statistically significant (n.s.) based on two-way ANOVA. N = 3 Error bars in this figure represent mean ± s. e.m.