TABLE 2.
Temperature and cycle settings for Real-Time PCR.
| Step | Cycles | Temperature (°C) | Duration |
|---|---|---|---|
| Pre-incubation | 1 | 95 | 10 min |
| Amplification | 50 | 95 | 15 s |
| 60 | 30 s | ||
| 72 | 30 s | ||
| Melting curve | 1 | 95 | 5 s |
| 50 | 1 min | ||
| 97 | Continuous | ||
| Cooling | 1 | 40 | 30 s |
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