Table 6.
Thiobarbituric acid reactive substances (TBARs), hydroperoxides (Hy), protein carbonyls, ferric reducing antioxidant power (FRAP), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) serum concentration in dog treated with dietary supplementation of PTHL and untreated dog (CON) for 90 days.
| Parameter | Group | Time (days) | SEM1 | p -value | |||||
|---|---|---|---|---|---|---|---|---|---|
| 0 | 30 | 60 | 90 | Group | Time | G × T2 | |||
| TBARs (mmol/mL) |
CON | 1.14 | 1.11 | 1.14 | 1.02 | 0.105 | 0.223 | 0.042 | 0.622 |
| PTHL | 1.39 | 1.37 | 1.16 | 1.13 | |||||
| Hydroperoxides (mmol/mL) |
CON | 5.18 | 5.27 | 5.75 | 4.53 | 0.495 | 0.554 | 0.307 | 0.836 |
| PTHL | 4.93 | 5.33 | 5.76 | 5.31 | |||||
| Protein carbonyls (μmol/mg Protein) |
CON | 98.95 | 91.26 | 92.06 | 93.47 | 2.630 | 0.050 | 0.192 | 0.667 |
| PTHL | 96.04 | 96.76 | 96.04 | 99.00 | |||||
| FRAP (μmol TE/mL) |
CON | 48.40 | 46.96 | 46.77 | 44.55X | 0.380 | <0.0001 | <0.0001 | <0.0001 |
| PTHL | 49.44 | 48.05 | 49.81 | 51.10Y | |||||
| SOD (U/mL) |
CON | 48.41A | 49.84X | 54.52X | 54.07X | 1.140 | 0.522 | <0.0001 | <0.0001 |
| PTHL | 20.39A | 60.05BY | 72.40CY | 91.91DY | |||||
| CAT (U/mL) |
CON | 2.88A | 3.21X | 3.33X | 3.28X | 0.125 | 0.320 | <0.0001 | <0.0001 |
| PTHL | 2.12A | 4.30BY | 4.24BY | 4.34BY | |||||
| GSPx (nmol NADPH ox/mL) |
CON | 2.43A | 2.43X | 2.52X | 2.63X | 0.035 | 0.106 | <0.0001 | <0.0001 |
| PTHL | 2.27A | 2.91BY | 2.96BY | 3.27BY | |||||
1
Standard error of the means.
2
Group × Time.
Different letters on the same line show statistical differences during time in the same group: A, B, C, D = p < 0.01; Different letters on the same row show statistical differences between groups at the same aging time: X, Y = p < 0.01.