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. 2022 Jun 1;13:919519. doi: 10.3389/fpls.2022.919519

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Copyright © 2022 King, Grewal, Othmeni, Coombes, Yang, Walter, Ashling, Scholefield, Walker, Hubbart-Edwards, Hall and King.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Breeding scheme used to generate homozygous wheat-Triticum timopheevii introgression lines (ILs) from advanced backcrossed lines generated by Devi et al. (2019). For each year (one generation per year), the number of families, Kompetitive allele specific PCR (KASP) marker sets assessed, selected plants, and type of cross are shown. The backcrosses with the recurrent parents are represented by an × and a wheat plant, and the self-fertilisation rounds by an encircled ×. In each year a set of homozygous ILs were selected for additional rounds of self-fertilisation to create a panel of plants carrying overlapping T. timopheevii segments of varying sizes.