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Aloperine directly affects Th17 in vitro differentiation by inhibiting the STAT3 signalling. (A–C) IFN-γ+ Th1, IL-4+ Th2, and IL-17A+ Th17 polarizing efficiency were analyzed by flow cytometry. (D) The levels of RORγt and phosphorylated STAT3 were assessed by western blotting. The results were from three independent experiments. The values are presented as the mean ± SEM. ∗p < 0 05; ∗∗p < 0.01; ∗∗∗p < 0.001; and ns, p ≥ 0.05.
