Fig. 1.

A Western blot of culture medium (cm) obtained from the pIZ-NA transfected Sf9 cells, prepared in non-reducing condition, using anti-NA monoclonal antibody. Lane M: marker proteins. B PCR of reverse transcription for NA gene expression analysis of transfected cells (T) and clone cells no.1–6 of the pIZ-NA transfected Sf9 cells. C PCR of reverse transcription for NA gene expression analysis of clone no.2 at passage no. 10, 20, 30, 40, and 50. − : negative control for PCR using non-transfected cells cDNA, + : positive control for PCR using pIZ-NA as a template