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. 2022 May 27;12:884411. doi: 10.3389/fcimb.2022.884411

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Copyright © 2022 Qiu, Xu, Liu, Ren, Han and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimal ssDNA probe and work concentration screening for the N. farcinica CRISPR-CLA assay. The 11 nt ssDNA probe with 250 nM work concentration was screened out, because of the highest fluorescence value. nt, nucleotide; NC, negative control; BC, blank control. (n = 3 technical replicates; bars represent mean ± s.e.m.).