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. 2022 May 27;12:884411. doi: 10.3389/fcimb.2022.884411

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Copyright © 2022 Qiu, Xu, Liu, Ren, Han and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Feasibility of the N. farcinica CRISPR-CLA assay using clinical samples. (A) The fluorescence N. farcinica CRISPR-CLA assay results. Sample S1 to S21 showed positive results and sample N1 to N20 showed negative. (n = 3 technical replicates; bars represent mean ± s.e.m.) (B) The lateral-flow N. farcinica CRISPR-CLA assay results. Sample S1 to S21 showed positive results and sample N1 to N20 showed negative. S1-S20, simulated positive samples; S21, positive sample; N1-N20, negative samples; PC, positive control; NC, negative control; BC, blank control.