Figure 3.

Phylogenetic analysis of 294 ORF2 fragment genetic sequences of HEV recovered in Spain, including two sequences of Andorra, 2013–2019 (n = 323 sequences in the tree)

HEV: hepatitis E virus; ORF2: open reading frame 2.

Potential HEV-3f-A1, HEV-3f-A2 and HEV-3f-B subclusters are shown with red, blue and green lines respectively. The 294 sequences obtained in this study are indicated with coloured circles, with colour according to year of sampling. Reference sequences of HEV genotypes 1, 2, 3, 4, 5, 6, 7 and 8 were included in the analysis. Genbank accession numbers of sequences used for the phylogenetic analysis are indicated on the tree leaves. Bootstrap values indicating the number of repetitions of the tree with the showed topology are included in the tree nodes defining clades, genotypes, subtypes, clusters and subclusters.