Fig. 1.

Experimental layout. A) Description of the experimental groups and timepoints for behavioral test, electrochemical analysis, perfusions, and dissections. One group was injected with PFFs only (PFFs), two groups received either AAV-h-αSyn or AAV-GFP (AAV), two groups received AAV-h-αSyn or AAV-GFP and PFFs simultaneously (Sim.), and two groups received AAV-h-αSyn or AAV-GFP and PFFs sequentially (Seq.). B) Schematic of the pre-formed fibrils and viral genome expressing h-αSyn or GFP under the CBA promoter injected into the midbrain of rats. ITR, inverted terminal repeat; CBA, chicken β-actin; pA, polyadenylation site; h-αSyn, human alpha-synuclein; GFP, green fluorescent protein; PFFs, preformed fibrils; MB, midbrain; ST, striatum.