Fig. 4.

Behavioral impairments following h-αSyn and/or PFFs injection. A-F) Behavioral tests performed to assess motor function at 4-, 8-, and 12-weeks post-injection for Cylinder, Stepping, and Rotation test for the h-αSyn (circles, A, C, E), GFP (squares, B, D, F), and PFF (circles, A-F) injected groups, respectively. G) Electrochemical recordings (chronoamperometry) for KCl-evoked striatal DA release; black arrow indicates the timepoint of KCl application. H) Average peak amplitude, reflecting the maximum amount of dopamine available, for all the seven experimental groups. PFFs, preformed fibrils; h-αSyn, human alpha-synuclein; GFP, green fluorescent protein; w, weeks; s, seconds. Data are expressed as mean ± SEM. Note: the behavioral results of the PFFs group are duplicated in each graph for ease of comparison.