Fig. 5.
h-αSyn overexpression and PFFs treatment results in Lewy-like midbrain pathology. A-Di) pSer129 immunoreactivity in midbrain before (-pK) and after (+pK) proteinase K treatment. E, F) TR-FRET quantification of pSer129 (E) and aggregated αSyn (F) in the ipsilateral midbrain and striatum of rats and cingulate cortex of human samples. Black dots represent single subjects. G-Jiii) Triple immunofluorescence showing co-localization between pSer129 and Thioflavin S. K-Niii) Triple immunofluorescence showing co-localization between 81A (pSer129) and Ubiquitin antibodies. PFFs, preformed fibrils; h-αSyn, human alpha-synuclein; pK, Proteinase K; Ub, ubiquitin; ThioS, Thioflavin S; ST, striatum; MB, midbrain; CC, cingulate cortex. (A.U. = ratio 665 nm/620 nm multiplied by 10000).