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. 2022 Feb 3;106(6):1083–1097. doi: 10.1093/biolre/ioac029

Figure 6.

Figure 6

(A) Immunofluorescence of E9.5 decidua tissue sections (mesometrial) showing colocalization of CD45 pan-leukocyte marker (white), GFP marker of transplanted BMDCs (green), and DBA marker of NK cells (red) in control and CXCR4KO mice. Sections were counterstained with DAPI for nuclear staining (blue). White arrows point to nonhematopoietic GFP+ BMDCs that are negative for either CD45 or DBA markers. Yellow arrows point to GFP + CD45 + DBA+ BM-derived NK cells. Purple arrows point to GFP + CD45 + DBA– BM-derived non-NK immune cells. Note that nonhematopoietic GFP+ BMDCs (GFP + CD4 – DBA–) are found in control mice (white arrows) but not in CXCR4KO mice, where all GFP+ BMDCs are hematopoietic (CD45+ and/or DBA+). Low magnification picture scale bar, 200 μm. High magnification picture scale bar, 50 μm. (B) Quantitative summary of percentage of DBA+ (NK), DBA– CD45+ (immune non-NK) and DBA-CD45- (nonhematopoietic) BMDCs out of total GFP+ cells in the implantation site (E9.5) of control and CXCR4KO mice. n = 4 mice/group, **P < 0.01. Data in bar graphs are shown as mean ± SEM.