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. 2022 May 12;17(1):57. doi: 10.3892/br.2022.1540

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Copyright: © Onodera et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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PrP^Sc inactivation evaluated by PMCA. Brain homogenate of CD-1 mice was used as PrP^Sc source to infect N2a cells, and PMCA buffer was used to dilute samples (dilution series are indicated above the blots). PMCA products from R9 of amplification were analyzed by western blotting after proteinase K digestion (2). (A) Control samples treated with PBS. (B) CAC-717-treated samples. Except for the NS, amplification was performed in quadruplicate. Molecular mass markers are indicated on the right-hand side. Cited from Sakudo et al (2) under the Created Commons Attribution 4.0 International license. PrP^Sc, scrapie prion; PMCA, protein misfolding cyclic amplification; R9, round 9; NS, non-seeded control.