Figure 2.

Effect of RAPA on iNOS and COX-2 expression in LPS-treated RAW 264.7 cells. Cells were retreated with the indicated concentrations of RAPA for 1 h and subsequently stimulated with 1 µg/ml LPS for 24 h. The expression of iNOS and COX-2 were determined by western blot analysis (A) and analyzed using ImageJ software (B) and (C). Data are presented as the mean ± SD. ^#P<0.05 vs. untreated cells; ^*P<0.05 vs. LPS treated cells. RAPA, red ginseng marc, Artemisia scoparia Waldst.et Kit, Paeonia japonica Miyabe & Takeda, and Angelica gigas Nakai extract mixture.