FIG. 3.

Southern hybridization detection of sec gene. Staphylococcal chromosomal DNA was restricted with RsaI and separated on a 2% (wt/vol) agarose gel. The DNA was hybridized at high stringency with digoxigenin-labeled, PCR-amplified toxin C gene. Lane 1, NCTC 10655 (SEC); lane 2, NCTC 8532 (nonenterotoxigenic); lanes 3 and 4, ovine strains. The two strongly hybridizing bands of 160 and 260 bp are those predicted from an analysis of the published gene sequence of sec (8). The fainter-hybridizing bands correspond in size to predicted partial restriction digest fragments. The relative positions of the molecular size markers are indicated on the left.