TABLE 2.
Toxin analysis of control strains by PCR and SET-RPLA toxin assays
| Test straina | Toxin serotype | PCR toxin type |
|---|---|---|
| S. aureus NCTC 8532 | − | − |
| S. aureus NCTC 10652b | SEA, SED | SEA, SED |
| S. aureus NCTC 10654 | SEB | SEB |
| S. aureus NCTC 10655 | SEC | SEC |
| S. aureus NCTC 10656 | SED | SED |
| S. aureus NCTC 10657 | SEA, SEB | SEA, SEB |
| S. aureus F287 | SEA | SEA |
| S. aureus FRI326 | SEA | SEE |
| S. aureus NCTC 11963 (TSST-1)b | SEC | SEC |
| S. chromogenes NCTC 10530 | − | − |
| S. epidermidis NCTC 12100 | SEC | SEC |
| S. hyicus NCTC 10350 | − | − |
| S. intermedius NCTC 11048 | − | − |
| S. lentus NCTC 12102 | − | − |
| S. saprophyticus NCTC 7292 | − | − |
| S. sciuri NCTC 12103 | − | − |
| S. warneri NCTC 11044 | − | − |
S. aureus strains which produce enterotoxins A (F287), B (NCTC 10654), C (NCTC 10655), D (NCTC 10656), and E (FRI326) and nonenterotoxigenic strain (NCTC 8532) were used as reference standards. Strain F287 is from the Nottingham Laboratory Collection and is known to produce enterotoxin A only. Nonstaphylococcal strains used to demonstrate the specificity of the PCR assay were Bacillus cereus, Bacillus subtilis, Corynebacterium oris, Enterobacter spp., Escherichia coli, Klebsiella pneumoniae, Lactococcus lactis, Lactobacillus spp., Listeria monocytogenes, Micrococcus luteus, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella spp., Shigella spp., and Yersinia enterocolitica.
The presence of toxins D and C in these strains was established by this study.