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. 2021 Nov 15;163(7):e882–e887. doi: 10.1097/j.pain.0000000000002535

Table 1.

Primers used for genomic DNA amplification and Sanger sequencing.

Amplicon size (bp) Oligo sequence (5′-3′) Ta (°C) Cycles n.
Exon 3 346 TGGAGTCTGATGGCAGTGTT—Fw
CCAGAGTCTTTCAAGGTGCAA—Rev		 57 30
Intron 2 508 ACTCTGACGTTCCTTCAACTT—Fw
CTGCAGGAGGAAAAAGAAAGGA—Rev		 56 30

Fw, forward primer; Rev, reverse Primer; Ta, annealing temperature; Cycles n., amplification cycles number.