Skip to main content
. 2021 Nov 15;163(7):e882–e887. doi: 10.1097/j.pain.0000000000002535

Table 2.

Primers used for cDNA amplification and Sanger sequencing.

Amplicon size (bp) Oligo sequence (5′-3′) Ta (°C) Cycles n.
Exon 2–4 311 AGTGACTTGGAAGCTGGCAA—Fw
TTTTTGGTCCAGTCCGGTGG—Rev		 57 35
Selective WT 364 TGCAGACAAAAAGACTTTCATAGT—Fw
TTCTGTTAAATACGCAAAAACAATGA—Rev		 57 35

Fw, forward primer; Rev, reverse primer; Ta, annealing temperature; Cycles n., amplification cycles number. The underlined letters indicate the splice junctions.