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. 2022 Jun 15;20:86. doi: 10.1186/s12964-022-00893-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — SOCS7 is downregulated by TGF-β1 and mediates TGF-β1-induced keratinocyte migration and wound healing. A, B. SOCS7 mRNA and protein levels in TGF-β1-treated HaCaT cells were analyzed by RT-PCR (A) and western blotting (B), respectively. C–E. HaCaT cells were transfected with Vector or SOCS7 plasmids, and scrambled siRNA (siR-ctrl) or SOCS7 siRNA (siR-SOCS7) for 24 h, then treated with TGF-β1 (10 ng/ml) for 24 h. SOCS7 protein levels were analyzed by western blotting (C). The scratch‐wound assay was performed to analyze the effect of SOCS7 on TGF‐β1‐dependent cell migration. Representative images (left) and quantification of data showing the percentage of wound closure (right) (D). The Transwell migration assay was carried out to determine the effect of SOCS7 on TGF‐β1-dependent cell migration. Representative images showing crystal violet staining (left) and quantification of data showing the number of migrated cells/field (right) (E). Data are given as mean ± standard deviation (SD) of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001