Fig. 2.

HCQ induces apoptosis of MDSCs and increases the level of CD81 in MDSCs. A Immunofluorescence detection of the apoptotic effects of HCQ on Gr-1⁺ cells in spleen. Scale bar = 50 μm (n = 3 mice/group). B The percentage of Annexin V⁺ in MDSCs in blood and spleen was determined by FACS (n = 6 mice/group). C The percentages of CD81⁺ in MDSCs were determined by FACS in blood and spleen (n = 6 mice/group). D Western blot was used to detect the protein expression of CD81 in purified MDSCs isolated from spleen (n = 4 mice/group). Data are presented as the mean ± standard error of the mean (SEM). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001