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. 2022 Jun 15;28:65. doi: 10.1186/s10020-022-00493-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — CD81 plays a crucial role in HCQ treatment by increasing the apoptosis of MDSCs. A, B The expression level of CD81 in MDSCs of blood and spleen was determined by FACS (A) and Western blotting (B) (n = 6 mice/group). C The number of MDSCs in blood and spleen was measured by FACs and compared with that of achieved statistically (n = 6 mice/group). D The percentage of Annexin V⁺ in MDSCs in blood and spleen was determined by FACs (n = 6 mice/group). Data are presented as the mean ± standard error of the mean (SEM). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001