Fig. 2.

SPINK5 modulates melanoma cells EMT by STAT3 phosphorylation. A Western blot and RT-qPCR were used to detect SPINK5 expression after transfection with either an siRNA or an expression vector. B, C Wound-healing and transwell assays were used to determine the effects of SPINK5 in melanoma cell metastasis. D SPINK5 modulates melanoma cell EMT process shown by EMT‑related markers determined by western blot analysis. E, F Immunoblotting and IF analysis of STAT3, and the phosphorylation of STAT3 in A2058 and G361 cell lines transfected with either a SPINK5 siRNA or expression vector. G, H After cotransfection, Co-IP was used to measure the interaction between SPINK5 and STAT3 in 293T cells. *p < 0.05, **p < 0.01, ***p < 0.001 versus control