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. 2022 Jun 15;27:48. doi: 10.1186/s11658-022-00353-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Metformin inhibits EMT by regulating the miR-5100/SPINK5/STAT3 axis. A IC₅₀ were detected in A2058 and G361 cells (16.61 and 15.1 mM, respectively). B, C Wound-healing and transwell assays were used to determine the effects of metformin in melanoma cell metastasis. D, E Western blot and RT-qPCR were used to detect miR-5100 and relative protein expression after treatment with metformin under a concentration of IC₅₀. F–H miR-5100 mimic reversed metastasis and EMT inhibition effect of metformin in melanoma cells partly. *p < 0.05, **p < 0.01, ***p < 0.001 versus control

Fig. 3 — Metformin inhibits EMT by regulating the miR-5100/SPINK5/STAT3 axis. A IC₅₀ were detected in A2058 and G361 cells (16.61 and 15.1 mM, respectively). B, C Wound-healing and transwell assays were used to determine the effects of metformin in melanoma cell metastasis. D, E Western blot and RT-qPCR were used to detect miR-5100 and relative protein expression after treatment with metformin under a concentration of IC₅₀. F–H miR-5100 mimic reversed metastasis and EMT inhibition effect of metformin in melanoma cells partly. *p < 0.05, **p < 0.01, ***p < 0.001 versus control