Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 15;20:87. doi: 10.1186/s12964-022-00898-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Knockdown of NEK2 leads to the impairment of migration and proliferation of ESCC in vitro. A The RNA-seq data of CCLE showed various expressions of NEK2 in ESCC. B The expression of NEK2 in ESCC was significantly higher than immortalized esophageal epithelia Het-1A in transcriptional and translational levels. C Both shRNA-2 and shRNA-3 in KYSE-510, as well as all strings in Eca-109, yielded efficacy in knocking down NEK2 at the translational level. D Knockdown of NEK2 in both cells showed declined migration in Transwell assay. E Knockdown of NEK2 in both cells exhibits decreased proliferation in clone formation assay. All experiments were carried out independently for three times. *P < 0.05, **P < 0.01, ***P < 0.001. CCLE Cancer Cell Line Encyclopedia