Fig. 5.

PIEZO1 expression is altered in AD-specific subpopulations of human and murine microglia. Piezo1 Log2 gene expression overlaid onto the Uniform Manifold Approximation and Projection (UMAP) plots of the clusters in A 5xFAD scRNA [41], B Trem2^−/− 5xFAD snRNA [42], and C human AD patient snRNA [40] dataset. Corresponding consensus scores summarizing the ranks of Piezo1 frequency and expression in respect to the other genes in all annotated clusters (see Additional file 1: Fig. S5): D for A data, E for B data and F for C data. Respective Piezo1 expression level in microglial subclusters of G WT and 5xFAD mice in (A), H all Trem2−/− 5xFAD subclusters in (B), and I in human microglial subtypes m1–m5 in (C). Subtypes m1 and m2 are only present in AD patients PIEZO1. Unpaired t-test, one-way ANOVA with Dunnett’s multiple comparisons, Kruskal–Wallis p.v. Significance *p < 0.05. Data as mean ± min and max. See also Additional file 1: Fig. S5