Figure 3.

Pyrosequencing to determine methylation frequency of the CpG sites in the CpG island containing the NFκB and CREB1 transcription factor consensus binding sites in the CMV promoter. a: Locations of the CpG sites evaluated and the relative locations of the transcription factor binding sites. b: Methylation fraction in A0 and A1 cell lines in control cell and azacytidine treated cultures. c: Methylation fraction in C0 and C1 cell lines in control cell and azacytidine treated cultures. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001, unpaired t-test.