Fig. 2.

DUSP3-deficient mice have decreased ZO-1 expression and a faster progression of EGFR mutant-driven lung adenocarcinoma. A Lung tissues derived from mice of different DUSP3 status were subjected to extract preparation and immunoblot analyses of proteins as indicated. The numbers showed the means and standard deviations of the signals. The means in DUSP3 +/+ mice were assigned as 100. B Lung tissue sections derived from DUSP3 +/+ and DUSP3−/− mice with or without an EGFR-Del transgenic gene were subjected to IF staining using a ZO-1 antibody. T and N indicated tumor and adjacent normal tissues, respectively. Hematoxylin and Eosin (H&E) staining of the consecutive sections showed the locations of the tumors. NC: negative control staining without the primary antibody. C Tumor incidences in 6-M and 12-M old male mice with indicated genotypes were analyzed by pathological examination of lung sections staining with hematoxylin and eosin. D The correlation between DUSP3 expression (RNA sequencing data) and LAC patient survival in the TCGA database was analyzed through the Kaplan Meier Plotter