Fig. 3.

DUSP3-deficiency is associated with increased OCLN phosphorylation in DelA cell lines. A Cell lysates collected from various DelA cell lines were subjected to 7% SDS-PAGE analyses containing 25 μM of Phos-Tag reagent plus MnCl₂, followed by immunoblot analyses using indicated antibodies. Phosphorylated OCLN with slower mobility was indicated by an arrowhead. B The expression and phosphorylation levels of Src were analyzed by immunoblot assays. The bar graphs showed the means and standard deviations of three independent experiments. The value of DelA-F11 was assigned as 1. * p < 0.05. C and D Confluent DelA-F13, F14R and F16R cells were subjected to a 24 h treatment of an FAK inhibitor (Defactinib; 5 μM) and the cells were collected and analyzed by immunoblot assays (C) or IF staining (D) using indicated antibodies