FIG. 1.

Nucleotide sequence of the L. lactis B1014 tributyrin esterase gene. The predicted amino acid sequence is given below the nucleotide sequence in the standard one-letter code. The stop codon is marked by an asterisk. The transcription initiation site mapped by primer extension is marked by a vertical arrow (nt 62). The putative Shine-Dalgarno site (SD), the conserved TG dinucleoside, and the −35 and −10 regions are underlined. The lipase/esterase consensus sequence at positions 119 to 123 is double underlined.