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. Author manuscript; available in PMC: 2022 Jun 15.
Published in final edited form as: J Vis Exp. 2022 Feb 11;(180):10.3791/63395. doi: 10.3791/63395

Figure 4: RT-qPCR detection of N1 (SARS-CoV-2) synthetic RNA and P1 (Hs_RPP30) synthetic DNA.

Figure 4:

Standard curves were plotted with standard deviations to determine the range of accurate detection using this probe/primer combination. (A) The mean Ct values (n =4) obtained in respective dilutions were plotted against the estimated quantity of synthetic RNA (1×100 to 1×104 RNA copies in 10 μL of RT-qPCR reaction). (B) The mean Ct values (n =3) obtained in respective dilutions were plotted against the estimated quantity of synthetic DNA (1 × 100 to 1 × 104 gene copies in 10 μL of RT-qPCR reaction).