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. Author manuscript; available in PMC: 2022 Jun 15.
Published in final edited form as: J Vis Exp. 2022 Feb 11;(180):10.3791/63395. doi: 10.3791/63395

Figure 5: Comparison between manual and automated saliva transfer SARS-CoV-2 (N1) Ct values.

Figure 5:

The known SARS-CoV-2 positive saliva samples (n =20) were loaded in duplicate into an RT-qPCR master mix plate by a liquid handling robot. The samples have a Ct value ranging from 18–32 for N1. The same samples were then manually loaded into duplicate wells in a different plate location. (A) N1 Ct values obtained from unique samples using both the robot and manual sample loading were transposed to determine inter-assay variability between manual and robot loading. (B) Intra-assay variability was also determined by using transposed replicate of N1 Ct values obtained from both robot and manual sample loading.