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. Author manuscript; available in PMC: 2022 Jun 15.
Published in final edited form as: J Vis Exp. 2022 Feb 11;(180):10.3791/63395. doi: 10.3791/63395

Table 1:

Components of N1+P1 probe/primer mix.

Component Sequence (5’→3’) Stock Concentration Volume
2019-nCoV-N1 Probe /5FAM/ACCCCGCAT/ZEN/TACGTTTGGTGGACC/3IABkFQ 50 μM 500 μL
2019-nCoV-N1-For GACCCCAAAATCAGCGAAAT 100 μM 2000 μL
2019-nCoV-N1-Rev TCTGGTTACTGCCAGTTGAATCTG 100 μM 2000 μL
Hs RPP30 Cy5 Probe /5Cy5/TTCTGACCT/ZEN/GAAGGCTCTGCGCG/3IABkFQ 50 μM 500 μL
Hs-RPP30-For AGATTTGGACCTGCGAGCG 100 μM 2000 μL
Hs-RPP30-Rev GAGCGGCTGTCTCCACAAGT 100 μM 2000 μL
Water - - 11000 μL