Table 2:

Components of multiplex SARS-CoV-2 master mix.

Component	Stock Concentration	Volume per reaction	Final Concentration	Batch Volume
Luna WarmStart RT Enzyme Mix	20X	0.5 μL	1X	3 mL
Luna Buffer Reaction Mix	2X	5.0 μL	1X	30 mL
N1+P1 Primer/Probe Mix	nCoV N1 F: 10 μM	0.5 μL	500 nM	3 mL
	nCoV N1 R: 10 μM		500 nM
	Probe nCoV N1: 2.5 μM		125 nM
	RPP_30 P1 F: 10 μM		500 nM
	RPP_30 P1 R: 10 μM		500 nM
	Probe RPP_30 P1: 2.5 μM		125 nM
Nuclease Free Water	---	2 μL	---	12 mL
Subtotal	---	8 μL	---	48 mL
Template		2 μL