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Touchdown RT-qPCR protocol.
| Stage | Temperature (°C) | Duration | Number of Cycles |
|---|---|---|---|
| Reverse Transcription | 55 | 10 min | 1 |
| Initial Denaturation | 95 | 1 min | 1 |
| Touchdown | 95 | 10 sec | 3 |
| 72 | 30 sec | ||
| 95 | 10 sec | 3 | |
| 69 | 30 sec | ||
| 95 | 10 sec | 3 | |
| 66 | 30 sec | ||
| Main Amplification | 95 | 10 sec | 40 |
| 65 | 30 sec |
Thermocycling conditions for one-step RT-qPCR SARS-CoV-2 diagnostic assay.
