| 100% EtOH |
Fisher scientific |
22-032-601 |
|
| 20 uL Filtered Pipette Tips |
Opentrons |
20uL tips |
|
| 2mL Microcentrifuge Tubes |
Fisher Scientific |
14-666-313 |
Alternate product may be used |
| Armadillo PCR Plate, 384-well, clear, white wells |
Thermo Scientific |
AB3384 |
Alternate product may be used |
| Celltreat 2mL 96 Deep Well Plates |
Fisher Scientific |
50-828-743 |
For mastermix preparation |
| Clear PCR Sealing Sheets |
Thermo Scientific |
AB0558 |
Alternate product may be used |
| DPEC Treated Water |
Ambion (Thermo Scientific) |
AM9916 |
|
| Flip Cap 50 mL Conical Tubes |
VWR |
75845-210 |
For sample collection |
| Foil PCR Sealing Sheets |
Thermo Scientific |
AB0626 |
For storage of mastermix plates, Alternate product may be used |
| HS_RPP30 Synthetic DNA |
Integrated DNA Technologies |
299788131 |
P1 positive control |
| Luna Buffer Probe One-Step Reaction |
New England Biolabs |
M3006B |
|
| Luna WarmStart RT Enzyme Mix |
New England Biolabs |
M3002B |
|
| nCOV_N1 Forward Primer, 100 nmol |
Integrated DNA Technologies |
10006830 |
|
| nCOV_N1 Probe Aliquot, 50 nmol |
Integrated DNA Technologies |
10006832 |
Probe can be synthesized by other vendors with SYBR or FAM fluophores |
| nCOV_N1 Reverse Primer, 100 nmol |
Integrated DNA Technologies |
10006831 |
|
| Opentron HEPA Filter Module |
Opentrons |
N/A |
Not required, but useful to reduce contamination |
| Opentron Multichannel Attachment, P20 |
Opentrons |
999-00005 |
For mastermix preparation |
| Opentron OT-2 Liquid Handling Robot |
Opentrons |
OT-2 |
|
| Opentron Pipette Attachment, P20 |
Opentrons |
999-0000215 |
For sample loading |
| Oven |
Memmert |
UF450 PLUS 208V-3PH |
|
| PCR Tubes (rnase, dnase free) |
Fisher Scientific |
14-230-225 |
For aliquots of positive and neg controls |
| PolarSafe Aluminum Cooling Block, 15-Well (1.5/2.0 mL Tubes) |
VWR |
10808-952 |
|
| PolarSaf Aluminum Cooling Block, 24-Well (0.5mL tubes) |
VWR |
10808-956 |
|
| RNAse P (ATTO 647) Probe, 50 nmol |
Integrated DNA Technologies |
10007062 |
Probe can be synthesized by other vendors with Cy5 fluorophore |
| RNAse P Forward Primer, 100nmol |
Integrated DNA Technologies |
10006836 |
|
| RNAse P Reverse Primer, 100 nmol |
Integrated DNA Technologies |
10006837 |
|
| Sars-CoV-2 Synthetic RNA Control 2 |
Twist Biosciences |
102024 / 103907 / 103909 |
N1 Positive Control |
| Scanners |
Code |
CR1500 |
Only required when scaling up |
| Small HEPA Filtered Hood |
Erlab |
Captair Bio 321 |
For mastermix preparation |
| Thermocycler CFX384 Touch |
Biorad |
CFX384 Touch |
Alternate models can be used, e.g. CFX384 Opus |
| X-acto Knife Set |
Staples |
N/A |
To cut foil for keeping control wells covered |
