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. Author manuscript; available in PMC: 2023 May 27.
Published in final edited form as: J Vis Exp. 2022 May 27;(183):10.3791/61504. doi: 10.3791/61504

Figure 1: Typical workflow.

Figure 1:

First, mice undergo a weight drop closed head injury, then cerebral blood flow (CBF) is measured using Diffuse Correlation Spectroscopy. Next, brains are collected, regions of interest are micro-dissected and snap frozen using liquid nitrogen. In preparation for the Luminex immunoassay, proteins are lysed, and total protein concentration is measured by BCA assay. Lysates are used for Western blot of proteins of interest and Luminex assays for cytokines and phospho-proteins. Data from CBF, Western blot, and Luminex are integrated using partial least squares regression (PLSR).