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. Author manuscript; available in PMC: 2023 May 27.
Published in final edited form as: J Vis Exp. 2022 May 27;(183):10.3791/61504. doi: 10.3791/61504

Table of Materials.

Name of Material/ Equipment Company Catalog Number Comments/Description
MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 32 Plex - Immunology Multiplex Assay Millipore Sigma MCYTMAG-70K-PX32
MILLIPLEX MAPL/SAPL Signaling 10-Plex Kit-Cell Signaling Multiplex Assay Millipore Sigma 48–660MAG
Bio-Plex cell lysis kit C Bio-Rad 171304012
Luminex sheath fluid EMD Millipore SHEATHFLUID
Luminex Drive Fluid Luminex MPXDF-4PK
BRAND BRANDplates pureGrade Microplates, Nonsterile BrandTech 781602 96
Tween20 Sigma-Aldrich P9416–50ML
Phosphate-buffered Saline (PBS) VWR 97064–158
DiH2O VWR VWRL0200–1000
Plate Sealer VWR 82050–992
Aluminum foil VWR 89107–726
Reagent Reservoirs VWR 89094–668
Polypropylene microfuge tubes VWR 20901–547
Phenylmethylsulfonyl fluoride Sigma-Aldrich P7626–1G
Complete mini protease inhibitor tablet Sigma-Aldrich 11836153001
Laboratory vortex mixer VWR 10153–838
Adjustable pipettes
Sonicator
Titer plate shaker VWR 12620–926
Mini LabRoller rotator VWR 10136–084
Luminex 200, HTS, FLEXMAP 3D, or MAGPIX with xPONENT software Luminex Corporation
Handheld magnetic separator block for 96 well flat bottom plates Millipore Sigma Catalogue 40–285
1 m acrylic guide tube McMaster-Carr 49035K85
54 g bolt Ace Hardware 0.95 cm basic body diameter, 2 cm head diameter, 10.2 cm length
Isoflurane 250 mL MED-VET INTERNATIONAL RXISO-250
Kimwipe (11.2 x 21.3 cm) VWR 21905–026
852 nm long-coherence length laser TOPTICA Photonics iBeam smart
4 photon counting avalanche photodiode Perkin-Elmer SPCM-AQ4C-IO
Hardware Autocorrelator Board www.correlator.com Flex05–8ch
LabView National Instruments LabVIEW
400 um multimode source fiber Thorlabs Inc. FT-400-EMT
780 nm single mode detector fiber Thorlabs Inc. 780HP
Depilatory cream Amazon Nair