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. 2022 Mar 9;40(5):523–536. doi: 10.1093/stmcls/sxac015

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© The Author(s) 2022. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 1. — Clustering and analysis of unsorted adult human ovarian cortical cells following scRNA-seq. (A) Identification of a total of 12 020 cells that formed 6 clusters following analysis of the dataset using Cell Ranger v2. (B) Identification of a total of 27 376 cells that formed 8 clusters following reassessment of the same dataset with Cell Ranger v3. (C) Cluster dendrogram depicting the lineage relationships between the 8 clusters identified using Cell Ranger v3. (D) Scatterplot analysis of the 62-cell germ cell/oocyte cluster identified using Cell Ranger v3, showing OSC gene expression scores plotted against oocyte gene expression scores. Two cells with very high OSC gene expression scores and an oocyte gene expression score of zero are highlighted by black arrows. (E) Identification of a total of 27 710 cells that formed 9 clusters following reassessment of the dataset with Cell Ranger v6. (F) Loupe Browser analysis of the Cell Ranger v6 output data, with the germ cell/oocyte cluster highlighted by the expanded box. Of the 62 cells in this cluster (each cell is depicted as an individual dot), 10 cells were identified as positive for co-expression of FIGLA, OOSP2, GDF9, and ZP3 (purple dots; examples are highlighted by black arrows) whereas 52 cells did not show co-expression of this 4-gene oocyte marker panel (light-gray dots; examples are highlighted by open arrowheads).