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. 2022 Mar 25;39(1):43–50. doi: 10.5511/plantbiotechnology.21.1225a

Figure 1. Effects of inhibitors of auxin biosynthesis in CIM culture on shoot regeneration in hypocotyl explants. (A) Hypocotyl explants cultured on SIM for 14 days after 4.5 days of CIM culture supplemented with the indicated concentrations of PPBo (left) or Kyn (right). Scale bars=1 cm. (B) Numbers of regenerated shoots (green) and adventitious roots (orange) in hypocotyl explants cultured on SIM for 10 days after 4.5 days of CIM culture with the indicated concentrations of PPBo (left) or Kyn (right). Mean values of 10 explants are shown with standard errors. Values with an asterisk(s) are significantly different from the control (0 µM) value at p<0.05 (*) or 0.01 (**) (Tukey’s test). (C) Hypocotyl explants cultured on SIM for 14 days after 14 or 21 days of CIM culture supplemented with and without 25 µM PPBo. The number of explants with regenerated shoots per the total number of explants is shown at the upper left corner of each photograph. The red arrowhead indicates a regenerated shoot bud. Scale bar=2 mm.

Figure 1. Effects of inhibitors of auxin biosynthesis in CIM culture on shoot regeneration in hypocotyl explants. (A) Hypocotyl explants cultured on SIM for 14 days after 4.5 days of CIM culture supplemented with the indicated concentrations of PPBo (left) or Kyn (right). Scale bars=1 cm. (B) Numbers of regenerated shoots (green) and adventitious roots (orange) in hypocotyl explants cultured on SIM for 10 days after 4.5 days of CIM culture with the indicated concentrations of PPBo (left) or Kyn (right). Mean values of 10 explants are shown with standard errors. Values with an asterisk(s) are significantly different from the control (0 µM) value at p<0.05 (*) or 0.01 (**) (Tukey’s test). (C) Hypocotyl explants cultured on SIM for 14 days after 14 or 21 days of CIM culture supplemented with and without 25 µM PPBo. The number of explants with regenerated shoots per the total number of explants is shown at the upper left corner of each photograph. The red arrowhead indicates a regenerated shoot bud. Scale bar=2 mm.