TABLE 2.
Specific activity in each G131 mutant subtilisin BPN′ measured by ABEAa
| Amino acid | Sp act | Character of amino acid |
|---|---|---|
| Phe | 2.6 | Aromatic |
| Arg | 2.1 | Basic |
| Met | 1.8 | S atom containing |
| Trp | 1.6 | Aromatic |
| Tyr | 1.5 | Aromatic |
| His | 1.5 | Basic |
| Ser | 1.5 | Polar |
| Asn | 1.4 | Amide containing |
| Asp | 1.4 | Acidic |
| Lys | 1.4 | Basic |
| Ala | 1.4 | Aliphatic |
| Thr | 1.3 | Polar |
| Gln | 1.3 | Amide containing |
| Glu | 1.3 | Acidic |
| Pro | 1.2 | Aliphatic |
| Cys | 1.2 | S atom containing |
| Ile | 1.1 | β-Branched aliphatic |
| Val | 1.1 | β-Branched aliphatic |
| Leu | 1.1 | Aliphatic |
| Gly (wild type) | 1.0 | Aliphatic and no side chain |
a
Relative activity was measured by the ABEA system described in detail in reference 17 using culture supernatant samples of subtilisins.