Fig. 3. Postimplantation death of Drp1^matΔ/+ embryos derived from Drp1^Δ/Δ oocytes.

Five-week-old Drp1^cKO and control Drp1^fl/fl females were mated with WT ^(+/+) males and assessed fetal development between E10.5 to E18.5 of pregnancy. (A) Uteri of 94% of plugged control Drp1^fl/fl females showed embryos as compared to only 60% of plugged Drp1^cKO females contained any embryo. Number of mice analyzed are shown. (B) Control females had an average of eight implants per pregnancy, whereas Drp1^cKO females had only four implants per pregnancy. Number of mice analyzed are shown. (C) Within confirmed pregnancies, there was a higher percentage of embryo resorption in Drp1^cKO females (76%) compared to only 3% in Drp1^fl/fl females. Number of mice analyzed are shown. While the uteri of E13.5 Drp1^fl/fl females showed normal developing embryos (D), the embryos in uteri of Drp1^cKO females were either dead (E, arrows) or severely growth retarded (E, arrowheads). Results show means ± SD (B and C). P value determined by chi-square test (A), two-tailed Student’s t test (B and C).