TABLE 1.
P. plecoglossicida strains and other bacterial strains used in this study and their motility and sensitivity to two phage isolates
| Straina | Isolation
|
Motilityb | EOPc
|
||
|---|---|---|---|---|---|
| Location | Year | PPpW-3 | PPpW-4 | ||
| Pseudomonas plecoglossicida strains | |||||
| FPC941d | Shiga Prefecture | 1994 | + | 0.9 | 1.2 |
| AK-9510ef | Kyoto Prefecture | 1995 | − | 1.4 | 2.0 |
| PH-9501ef | Hiroshima Prefecture | 1995 | − | 1.2 | 1.0 |
| PY-9701 | Yamanashi Prefecture | 1997 | − | 1.1 | 1.4 |
| PM-9801 | Miyazaki Prefecture | 1998 | − | 2.0 | 2.3 |
| FPC951d | Tokushima Prefecture | 1994 | + | 1.0 | 1.8 |
| PT-97002f | Tokushima Prefecture | 1997 | + | 1.0 | 5.8 |
| PT-97034f | Tokushima Prefecture | 1997 | − | 1.0 | 3.5 |
| PT-98028f | Tokushima Prefecture | 1998 | − | 1.3 | 0.8 |
| PT-98058f | Tokushima Prefecture | 1998 | + | 1.0 | 2.4 |
| PTH-9801f | Tokushima Prefecture | 1998 | + | 1.2 | 1.0 |
| PTH-9802 | Tokushima Prefecture | 1998 | − | 1.0 | 1.0 |
| PTH-9805 | Tokushima Prefecture | 1998 | − | 4.7 | 6.7 |
| PTH-9811 | Tokushima Prefecture | 1998 | − | 3.6 | 1.2 |
| PTH-9817 | Tokushima Prefecture | 1998 | + | 4.2 | 3.3 |
| PTH-9824 | Tokushima Prefecture | 1998 | − | 2.9 | 5.8 |
| PTH-9901 | Tokushima Prefecture | 1999 | − | 1.3 | 0.8 |
| PTH-9902 | Tokushima Prefecture | 1999 | − | 1.3 | 1.1 |
| H3-16 | Wakayama Prefecture | 1991 | + | 1.5 | 4.8 |
| 92P3-0403 | Wakayama Prefecture | 1992 | + | 1.5 | 2.8 |
| 93P1-0225 | Wakayama Prefecture | 1993 | + | 2.0 | 2.3 |
| 94P1-0104 | Wakayama Prefecture | 1994 | + | 2.1 | 3.3 |
| 95P7-0502 | Wakayama Prefecture | 1995 | − | 1.8 | 3.3 |
| 96P25-0729 | Wakayama Prefecture | 1996 | − | 1.3 | 2.2 |
| 97P18-0617 | Wakayama Prefecture | 1997 | − | 1.4 | 4.1 |
| 98P3-0127 | Wakayama Prefecture | 1998 | − | 1.5 | 2.2 |
| 98P4-1015 | Wakayama Prefecture | 1998 | − | 1.7 | 3.3 |
| Aeromonas hydrophila ET-79059 | <10−9 | <10−9 | |||
| Aeromonas salmonicida ATCC 14174 | <10−9 | <10−9 | |||
| Edwardsiella tarda NUF251 | <10−9 | <10−9 | |||
| Pseudomonas putida ATCC 12633 | <10−9 | <10−9 | |||
| Pseudomonas fluorescens 03L | <10−9 | <10−9 | |||
| Pseudomonas anguilliseptica NCMB1949 | <10−9 | <10−9 | |||
| Vibrio anguillarum PT-24 | <10−9 | <10−9 | |||
| Vibrio ordalii PT-81024 | <10−9 | <10−9 | |||
ATCC, American Type Culture Collection; NCMB, National Collection of Marine Bacteria (United Kingdom).
Motility was determined by the standard wet mount method.
The number of PFU was determined after 1 day of incubation at 25°C, and the EOP was quantified by calculating the ratio of PFU obtained with each strain to those obtained with strain PTH-9802.
See reference 14.
This strain was used as an indicator strain for phage isolation.